Journal: Frontiers in Oncology

Article Title: Microarray and bioinformatic analysis reveal the parental genes of m6A modified circRNAs as novel prognostic signatures in colorectal cancer

doi: 10.3389/fonc.2022.939790

Figure Lengend Snippet: Differentially expressed m6A modified circRNAs and mRNA. (A) Hierarchical clustering heatmap of differentially m6A modified circRNAs. (B) Volcano plots of differentially mRNA based on TCGA-COAD. (C) Volcano plots of differentially mRNA based on GSE106582. (D) The intersected genes between microarray, COAD-TCGA and GSE106582.

Article Snippet: Arraystar m6A-circRNA epitranscriptomic microarray was used to identify differentially m6A modified circRNAs between CRC and the control group.

Techniques: Modification, Microarray

Journal: Frontiers in Oncology

Article Title: Microarray and bioinformatic analysis reveal the parental genes of m6A modified circRNAs as novel prognostic signatures in colorectal cancer

doi: 10.3389/fonc.2022.939790

Figure Lengend Snippet: Information on circRNAs formed by nine parental genes.

Article Snippet: Arraystar m6A-circRNA epitranscriptomic microarray was used to identify differentially m6A modified circRNAs between CRC and the control group.

Techniques:

Journal: Frontiers in Genetics

Article Title: m6A Methylation in Cardiovascular Diseases: From Mechanisms to Therapeutic Potential

doi: 10.3389/fgene.2022.908976

Figure Lengend Snippet: m6A sequencing and microarray data in GEO related to CVD.

Article Snippet: GSE159309 , GPL25916 Arraystar Rat Epitranscriptomic microarray , m6A mRNA epitranscriptomic microarray , China , 18 Aug 2021 , Rattus norvegicus , m6A-modified transcripts between a control group and an lipopolysaccharide (LPS)-induced septic cardiomyopathy group , Septic cardiomyopathy.

Techniques: Sequencing, Microarray, Next-Generation Sequencing, Methylation, Control, Dissection

Journal: Journal of Molecular Cell Biology

Article Title: METTL3-mediated m 6 A modification of HMGA2 mRNA promotes subretinal fibrosis and epithelial–mesenchymal transition

doi: 10.1093/jmcb/mjad005

Figure Lengend Snippet: METTL3-mediated m 6 A modification enhances the stability of HMGA2 mRNA. ( A ) MeRIP–qRT-PCR with anti-m 6 A antibody was performed to determine the m 6 A enrichment of HMGA2 mRNA with or without METTL3 knockdown in primary RPE cells. ( B ) Western blotting analysis showed that HMGA2 protein level was induced by TGF-β2 and inhibited upon METTL3 knockdown. ( C ) Immunofluorescence analysis confirmed the reduced expression of HMGA2 in METTL3-knockdown RPE cells ( n = 15 for each group). Actin cytoskeleton was visualized by phalloidin staining, which targets F-actin. Scale bar, 25 µm. ( D ) Schematic representation of pmirGLO dual-luciferase vectors fused with WT or MUT HMGA2 3′UTR. ( E ) 3′UTR WT or 3′UTR MUT reporters were transfected into RPE cells along with empty vectors or METTL3 expression plasmid. Relative luciferase activity was measured after 48 h. ( F ) After transfection with either si-METTL3 or si-NC for 48 h, primary RPE cells were treated with Act-D to block transcription. HMGA2 mRNA was analyzed at the indicated times. Data present mean ± SD of three independent experiments. Student's t -test for two independent groups, one-way ANOVA tests for luciferase reporter assay and repeated measures, two-way ANOVA tests for HMGA2 mRNA decay experiment, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: According to the binding site provided by m 6 A epitranscriptomic microarray, the corresponding HMGA2 3′UTR (114730–113789), either WT or MUT (mutated A of the m 6 A sites with G), was cloned into the downstream of the pmirGLO dual-luciferase vector (Promega).

Techniques: Modification, Quantitative RT-PCR, Western Blot, Immunofluorescence, Expressing, Staining, Luciferase, Transfection, Plasmid Preparation, Activity Assay, Blocking Assay, Reporter Assay